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Whether all samples identified as positive by mPCR are truly positive in an aetiological perspective regarding LRTI remains to be evaluated in a well-defined patient material. The Binax NOW immunochromatographic test ICT detecting the pneumococcal C polysaccharide and a serotype-specific latex agglutination LA test detecting 23 pneumococcal capsular antigens were evaluated for establishing pneumococcal etiology in community-acquired pneumonia CAP by use of nonconcentrated urine. It can also be useful in BAL samples. The method was tested on samples from six patients with lower respiratory tract infection LRTI and H. För att få tillgång tilllämpliga referensmetoder utvecklade vi en indirekt immunafluorescens-metod för H. When the protocol was tested on bacterial strains belonging to 37 different species, no false negatives and only one false positive were noted. An mPCR was constructed for simultaneous identification of Streptococcus pneumoniaeHaemophilus influenzaeMycoplasma pneumoniaeand Chlamydophila pneumoniae.

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As such, these assays might have implications for antibiotic use in CAP.

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One LRTI patient and the two asymptomatic carriers showed stable antibody levels against their own isolate. In order to create appropriate reference standards, an indirect immunofluorescence test for H. LA has promising potential for pneumococcal serotyping, although further evaluation is required. The urinary antigen tests can reliably establish pneumococcal pneumonia. In conclusion, using nonconcentrated urine and dividing ICT-positive results into strongly and weakly positive results is a suitable way of performing ICT.

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